LOCUS       sequence-72451-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF001
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        51..431
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactagt ttttcaaatc
       61 agtgcgcaag acgtgacgta agtatccgag tcagttttta tttttctact aatttggtcg
      121 tttatttcgg cgtgtaggac atggcaaccg ggcctgaatt tcgcgggtat tctgtttcta
      181 ttccaacttt ttcttgatcc gcagccatta acgacttttg aatagatacg ctgacacgcc
      241 aagcctcgct agtcaaaagt gtaccaaaca acgctttaca gcaagaacgg aatgcgcgtg
      301 acgctcgcgg tgacgccatt tcgccttttc agaaatggat aaatagcctt gcttcctatt
      361 atatcttccc aaattaccaa tacattacac tagcatctga atttcataac caatctcgat
      421 acaccaaatc gggtaccaac aatgagccca gaacgacgcc cggccgacat ccgccgtgcc
      481 accgaggcgg acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg
      541 gtcaacttcc gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg
      601 cgggagcgct atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac
      661 gcgggtccct ggaaggcacg caacgcctac gactggacgg ccgagtccac cgtgtacgtc
      721 tccccccgcc accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc
      781 ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc
      841 gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc
      901 aagcacggga actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtgccg
      961 ccccgtccgg tcctgcccgt ca
//
