LOCUS       sequence-72453-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a D-alanine resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF003
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        51..431
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactagt ttttcaaatc
       61 agtgcgcaag acgtgacgta agtatccgag tcagttttta tttttctact aatttggtcg
      121 tttatttcgg cgtgtaggac atggcaaccg ggcctgaatt tcgcgggtat tctgtttcta
      181 ttccaacttt ttcttgatcc gcagccatta acgacttttg aatagatacg ctgacacgcc
      241 aagcctcgct agtcaaaagt gtaccaaaca acgctttaca gcaagaacgg aatgcgcgtg
      301 acgctcgcgg tgacgccatt tcgccttttc agaaatggat aaatagcctt gcttcctatt
      361 atatcttccc aaattaccaa tacattacac tagcatctga atttcataac caatctcgat
      421 acaccaaatc gggtaccaac aatgcactcg cagaagcgcg tcgttgtcct cggatcaggc
      481 gttatcggtc tgagcagcgc cctcatcctc gctcggaagg gctacagcgt gcatattctc
      541 gcgcgcgact tgccggagga cgtgtcgagc cagactttcg cttcaccttg ggctggcgcg
      601 aattggacgc ctttcatgac gcttacagac ggtcctcgac aagcaaaatg ggaagaatcg
      661 actttcaaga agtgggtcga gttggtcccg acgggccatg ccatgtggct caaggggacg
      721 aggcggttcg cgcagaacga agacggcttg ctcgggcact ggtacaagga catcacgcca
      781 aattaccgcc ccctcccatc ttccgaatgt ccacctggcg ctatcggcgt aacctacgac
      841 accctctccg tccacgcacc aaagtactgc cagtaccttg caaga
//
