LOCUS       sequence-72454-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a D-alanine resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF004
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     832..852
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        841..887
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactagt gtccccagat
       61 tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc
      121 acgtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa
      181 gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga
      241 tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa
      301 accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc
      361 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
      421 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      481 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      541 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      601 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      661 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      721 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      781 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      841 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaac
//
