LOCUS       sequence-72458-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a sulfadiazine resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF012
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        55..435
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactagg atccttttca
       61 aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt tttatttttc tactaatttg
      121 gtcgtttatt tcggcgtgta ggacatggca accgggcctg aatttcgcgg gtattctgtt
      181 tctattccaa ctttttcttg atccgcagcc attaacgact tttgaataga tacgctgaca
      241 cgccaagcct cgctagtcaa aagtgtacca aacaacgctt tacagcaaga acggaatgcg
      301 cgtgacgctc gcggtgacgc catttcgcct tttcagaaat ggataaatag ccttgcttcc
      361 tattatatct tcccaaatta ccaatacatt acactagcat ctgaatttca taaccaatct
      421 cgatacacca aatcgccatg gcttctatga tatcctcttc agctgtgact acagtcagcc
      481 gtgcttctac ggtgcaatcg gccgcggtgg ctccattcgg cggcctcaaa tccatgactg
      541 gattcccagt taagaaggtc aacactgaca ttacttccat tacaagcaat ggtggaagag
      601 taaagtgcat ggtgacggtg ttcggcattc tgaatctcac cgaggactcc ttcttcgatg
      661 agagccggcg gctagacccc gccggcgctg tcaccgcggc gatcgaaatg ctgcgagtcg
      721 gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg cctgtatcgc
      781 cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat cagatgcacc
      841 gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag cgcggcgtgg
      901 gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat attgct
//
