LOCUS       sequence-72459-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides an F-H adapter as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGG001
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(89..108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(90..108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(118..140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(334..353)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     453..475
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(513..531)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        599..703
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaactatg agtaggcaag
       61 atgttctgta ggtgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      301 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      361 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      421 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      481 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      541 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      841 actggatctc aacagcgg
//
