LOCUS       sequence-72461-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides linker:BFP as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD005
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(884..903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(885..903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcatcaggt ggatcaggtt
       61 ctggtagtgg ttctggttca ggaggtacct ctggatctgg atcaggttct ggtagtggtt
      121 ctggttcagg atctgcatgc atggtttcaa agggtgagga actcatcaaa gaaaacatgc
      181 acatgaagct ctacatggaa ggtactgtgg ataaccacca tttcaagtgc acatctgaag
      241 gtgagggaaa gccttatgag ggaactcaga ccatgagaat caaggttgtg gaaggtggac
      301 ctcttccttt cgctttcgat atccttgcta cctcattcct ctacggatct aagaccttca
      361 tcaaccacac tcagggtatc cctgatttct tcaagcaatc tttcccagag ggattcactt
      421 gggagagagt tactacttac gaggatggtg gtgttctcac tgctactcag gatacttctc
      481 ttcaggatgg atgcctcatc tacaacgtga agatcagagg tgtgaacttc acctctaacg
      541 gacctgtgat gcagaaaaag actcttggat gggaggcttt cactgagaca ctttatcctg
      601 ctgatggtgg acttgagggt agaaacgata tggctcttaa gctcgttgga ggatctcacc
      661 ttatcgctaa catcaagacc acctacaggt ctaagaagcc tgctaagaac cttaagatgc
      721 ctggtgtgta ctatgtggat tacaggcttg agaggatcaa agaggctaac aacgagactt
      781 acgttgagca gcatgaagtt gctgttgcta gatatgctgg acttggaggt ggacttaatg
      841 gaatggatga actttacaag tagctgctga gaccgaattc tcgccctata gtgagtcgta
      901 ttacctggcc gtcgttttac aacgtcgtga ctgg
//
