LOCUS       sequence-72462-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the At1g04880 terminator as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE003
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(576..595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(577..595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(605..627)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(821..840)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcactgcag attcttctct
       61 tgtttcttac tccccgttgc cagtttcgtg ttccataggt aaatgttaga gagaggaaag
      121 ggcttcagat accttgcttc tggcccaaga ttatgtcttt ttggtttttt ttttggtttt
      181 tttggatttt tcttcggaaa caaggcaagt ttatggttat aacattttgg cataagagaa
      241 ttttttactt tcgttattaa tgggatatcc tgtaaattgg tgattaaaat ctgaaagggc
      301 cttataaact tgggcttgga aagcaaatgg gccgaagaag taatccctgt aatatgattt
      361 tgaaagcgga ggagggagac ttaaagactt tgattgattg atttataaaa ggaaccctaa
      421 gtttccgaac tttgtccgtc gccgtctgaa aactctgtag ctctctcact tcagctttcc
      481 aattaaaggg aacaaagctt tgatttttgc cgcagtcaga agcattactt tgcaagaaca
      541 agtgtgtaaa caaagactat gagaccgaat tctcgcccta tagtgagtcg tattacctgg
      601 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      661 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      721 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      781 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      841 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      901 tgctcccggc atccgcttac aga
//
