LOCUS       sequence-72463-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the At1g76110 terminator as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE005
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(612..631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(613..631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(641..663)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(857..876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcactgcgt gatttagtta
       61 aaacggcaag tcgttttgtt gtaggtcgtt tctctctttt aagattggac gcctcgccgt
      121 ttcttttatt ttctagctta ttgtagttgt ccatgcttgc ttaactaggc tcagtgagag
      181 gctagacttg ccaaacttga agagtcttta tttatttaag aattttagat gttctaatgt
      241 attaatcctt tttcgtaaac ctttcctgtt aaacaagagg tttatgttgc atgttaaaaa
      301 agaggtttag gttatattag caatttagca ttcgattcgt tggttagtaa ctaagtaata
      361 taaataccac aagaacaaga aatactaatg aaaacttgga atgttcaaag tctccttatc
      421 tacttatgca acgttagaca aagctatcaa acaatgccac gtcacctcac aaaacatcta
      481 gggtaaccaa tcacatgcag ataactctct cgctttattt tctcttcaaa tcttatcaaa
      541 aatcccataa tgttttcatt tctttacaag tgatcaacaa aacaccccaa aactatgaga
      601 ccgaattctc gccctatagt gagtcgtatt acctggccgt cgttttacaa cgtcgtgact
      661 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      721 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      781 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      841 tatggtgcac tctcagtaca atctgctctg atgccgca
//
