LOCUS       sequence-72472-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate N-terminal tag + coding 
            sequence + C-terminal tag modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGI000
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             88..747
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(154..173)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cacgagctat ttaggtgaca ctatagaagt gaagcttggt ctcaaacagt ggatccagtg
       61 ctaacatggt ctagaaggag gtcagctatg gagaaaaaaa tcactggata taccaccgtt
      121 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      181 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      241 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      301 gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      361 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      421 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      481 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
      541 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
      601 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
      661 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
      721 tgcgatgagt ggcagggcgg ggcgtaagag ctcaggagga cagctatgca gtttaaggtt
      781 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
      841 gacacgcccg ggcgacgga
//
