LOCUS       sequence-72474-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA left border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGY003
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    146..170
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     312..331
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     525..547
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(577..882)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(679..698)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgagatc
      121 tcaaacaaac acatacagcg acttagttta cccgccaata tatcctgtca aggctcgaac
      181 taggctcgga cgaagtaagc ggccgcaacc ttaattaaac acggcgcgcc aacagcgatc
      241 gcaccaggta ccaccttgag accgaattca aaaaaaagcg gacctccgaa gaggtccgcc
      301 agatgatcat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
      361 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
      421 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      481 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      541 taaaacgacg gccagcattg attacctcct actagtttat attccccaga acatcaggtt
      601 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      661 ggagactggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      721 caccactggg taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg
      781 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
      841 ataacggctc tctcttttat aggtgtaaac cttaaactgc atagctgtcc tcctgagctc
      901 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
//
