LOCUS       sequence-72475-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA right border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGY001
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    147..171
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     275..294
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     488..510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(540..845)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(642..661)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgagat
      121 ctcaaacaaa cacatacagc gacttagttt acccgccaat atatcctgtc aaggggatct
      181 ggctgagaac gccagctgtg catgcttggt ctagaatact gagaccctcg agaaaaaaaa
      241 gcggacctcc gaagaggtcc gccagatgat catgcggcat cagagcagat tgtactgaga
      301 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      361 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      421 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      481 gggttttccc agtcacgacg ttgtaaaacg acggccagca ttgattacct cctactagtt
      541 tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga
      601 tcagccactt cttccccgat aacggagact ggcacactgg ccatatcggt ggtcatcatg
      661 cgccagcttt catccccgat atgcaccact gggtaaagtt cacgggagac tttatctgac
      721 agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca
      781 ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac
      841 tgcatagctg tcctcctgag ctcttacgcc ccgccctgcc actcatcgca gtactgttgt
      901 aattcattaa gcattctgcc gac
//
