LOCUS       sequence-72477-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate supermodules with plant 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGN000
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..820
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(227..246)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttacttaag ctcgggcccg gtctcatagg gaattcgctt actaaaagcc agataacagt
       61 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
      121 gtatgtcaaa aagaggtgtg ctaagcttag gaggtcagct atggagaaaa aaatcactgg
      181 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      241 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      301 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      361 gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      421 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      481 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      541 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
      601 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      661 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
      721 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
      781 attacaacag tactgcgatg agtggcaggg cggggcgtaa gagctc
//
