LOCUS       sequence-72478-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA right border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGZ001
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    146..170
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     274..293
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     487..509
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(539..844)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(641..660)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgagatc
      121 tcaaacaaac acatacagcg acttagttta cccgccaata tatcctgtca aggggatctg
      181 gctgagaacg ccagctgtgc atgcttggtc tagaatactg agaccctcga gaaaaaaaag
      241 cggacctccg aagaggtccg ccagatgatc atgcggcatc agagcagatt gtactgagag
      301 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
      361 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      421 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      481 ggttttccca gtcacgacgt tgtaaaacga cggccagcat tgattacctc ctactagttt
      541 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      601 cagccacttc ttccccgata acggagactg gcacactggc catatcggtg gtcatcatgc
      661 gccagctttc atccccgata tgcaccactg ggtaaagttc acgggagact ttatctgaca
      721 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
      781 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      841 gcatagctgt cctcctgagc tcttacgccc cgccctgcca ctcatcgcag tactgttgta
//
