LOCUS       sequence-7250-ex        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Cudre-Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono 
            D
  TITLE     Lentivector-mediated transfer of Bmi-1 and telomerase in muscle 
            satellite cells yields a duchenne myoblast cell line with long-term 
            genotypic and phenotypic stability.
  JOURNAL   Hum Gene Ther. 2003 Nov 1. 14(16):1525-33.
  PUBMED    14577914
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2003 Nov 1. 14(16):1525-33."
COMMENT     Sequence Label: pLOX-CW-CRE
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             4..637
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    684..809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2043..2346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2347..2550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2501..2521
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2547..2571
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2643..2663
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2664..3692
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    3718..4306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3771..3791)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4189..4200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4450..4483)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4503..4683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4708..4726)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4709..4726)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5016..5035)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5249..5268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5368..5390
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5428..5446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5514..5618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5619..5687,5688..6479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5837..5856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6650..7238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7139..7158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7392..7409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7479..7499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7484..7813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7664..7799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8947..9012
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9142..9162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9587..9721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9624..9643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9678..9697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
       61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
      181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
      241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
      301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
      361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
      421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
      481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc
     2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctacca
     2641 tgcccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat
     2701 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
     2761 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
     2821 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
     2881 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
     2941 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt
     3001 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
     3061 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc
     3121 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta
     3181 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
     3241 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
     3301 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
     3361 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
     3421 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
     3481 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
     3541 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
     3601 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
     3661 caatggtgcg cctgctggaa gatggcgatt aggtcgagct caagcttcga attcgataat
     3721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcgag
     4321 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4381 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc
     4441 accatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta
     4501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4681 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     4741 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     4801 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     4861 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     4921 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     4981 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     5041 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     5101 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     5161 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     5221 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7021 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7441 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     7501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7561 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7621 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7801 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     7861 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     7921 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     7981 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     8041 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     8101 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     8161 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     8221 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     8281 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     8341 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     8401 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     8461 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     8521 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     8581 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     8641 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     8701 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     8761 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     8821 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     8881 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     8941 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9001 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     9061 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     9121 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     9181 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     9241 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     9301 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9361 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     9421 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     9481 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9541 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9721 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9781 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9841 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagt
//
