LOCUS       sequence-72509-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the rbcS terminator as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE001
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(14..655)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(679..697)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(680..697)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    711..727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(735..741,742..759,760..765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    780..801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtctcatagt cgattgatgc atgttgtcaa tcaattggca agtcataaaa tgcattaaaa
       61 aatattttca tactcaacta caaatccatg agtataacta taattataaa gcaatgatta
      121 gaatctgaca aggattctgg aaaattacat aaaggaaagt tcataaatgt ctaaaacaca
      181 agaggacata cttgtattca gtaacatttg cagcttttct aggtctgaaa atatatttgt
      241 tgcctagtga ataagcataa tggtacaact acaagtgttt tactcctcat attaacttcg
      301 gtcattagag gccacgattt gacacatttt tactcaaaac aaaatgtttg catatctctt
      361 ataatttcaa attcaacaca caacaaataa gagaaaaaac aaataatatt aatttgagaa
      421 tgaacaaaag gaccatatca ttcattaact cttctccatc catttccatt tcacagttcg
      481 atagcgaaaa ccgaataaaa aacacagtaa attacaagca caacaaatgg tacaagaaaa
      541 acagttttcc caatgccata atactcaaac tcagtaggat tctggtgtgt gcgcaatgaa
      601 actgatgcat tgaacttgac gaacgttgtc gaaaccgatg atacgaacga aagctttgca
      661 gtgagaccaa gcttcacttc tatagtgtca cctaaatagc tcgtgtgaaa ttgttatccg
      721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      841 ctgtcgtgcc agctgcatta atga
//
