LOCUS       sequence-72513-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF002
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(246..797)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctatagggcg agaattcggt ctcaatactc actggatttt ggttttagga attagaaatt
       61 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
      121 acatgagcga aaccctataa gaaccctaat tcccttatct gggaactact cacacattat
      181 tctggagaaa aatagagaga gatagatttg tagagagaga ctggtgattt ttgcggactg
      241 agctctcaga tttcggtgac gggcaggacc ggacggggcg gcaccggcag gctgaagtcc
      301 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg
      361 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg
      421 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag
      481 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtatacggt ggactcggcc
      541 gtccagtcgt aggcgttgcg tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg
      601 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc
      661 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg
      721 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg
      781 cgtcgttctg ggctcattg
//
