LOCUS       sequence-72517-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ozturk N, VanVickle-Chavez SJ, Akileswaran L, Van Gelder RN, Sancar 
            A
  TITLE     Ramshackle (Brwd3) promotes light-induced ubiquitylation of 
            Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4980-5. doi: 
            10.1073/pnas.1303234110. Epub 2013 Mar 11.
  PUBMED    23479607
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1303234110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "4980-5"
COMMENT     Sequence Label: pcDNA3.BRWD3-V5H
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(96..137)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aggtgaacta tagaataggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga
       61 taaactcaat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc gaggagaggg
      121 ttagggatag gcttaccttc gaagggccct ctagactcga gcggccgcca ctccttgaag
      181 ataccgcgcg ccttggcaga cggcttgcgc accctgcctc tgctgctaac gttcacctgc
      241 tgttcttccg agtccaccga agtaccgtcc tcttcttcct cctggtcccg ctcggtggag
      301 gcctgtgatc gctcctgaga gctggcatga gcgtcgccgt tacggattcg tttactccga
      361 tgggaatatc cagccgccgc ctcttcctcc tcggactgct cattgtagcg cggtctcttt
      421 tggttgcgcc cggctcgctg gtttccaccc cggttggcgc gacgactccg cggcacaaca
      481 tagtcatcat cgtcatcgtt gtcgttcgcg atggagttat tggatggcgg gcgacgtgtg
      541 cgtcgcagcg gacgctcctg agttgttgcg gaggaggagg aggtttttgc ctttcgcctc
      601 ggacgaccat tgctgggcga tacgtagctg gtcagcggct gattgtcttc cgagtcggag
      661 tcttcatcct catcttcgta gctctgcgag ctagagcctc catcagtgcc ctcatcggaa
      721 gcagcagcct cctcttcctc atgatcctcg tcgtgcgaat tcgcctggct agcgcgacca
      781 cgcagacgcc ttgtaaccac gttgctcgca ctgttgctgt tcgtggtaga ggtgggtcgc
      841 cggtgattgg ctatggctga
//
