LOCUS       sequence-72524-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with PhiYellow with 
            SpHis5 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     Yeast tagging plasmids.
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-link-PhiYellow-SpHis5
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..734
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="SSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQF
                     ICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNFK
                     TRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAFK
                     ICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKET
                     VRAVDCRKTYL"
ORIGIN
        1 gacggatcgg tgacggtgct ggtttaatta actccagcgg cgccctgctg ttccacggca
       61 agatccccta cgtggtggag atggagggca atgtggatgg ccacaccttc agcatccgcg
      121 gcaagggcta cggcgatgcc agcgtgggca aggtggatgc ccagttcatc tgcaccaccg
      181 gcgatgtgcc cgtgccctgg agcaccctgg tgaccaccct gacctacggc gcccagtgct
      241 tcgccaagta cggccccgag ctgaaggatt tctacaagag ctgcatgccc gatggctacg
      301 tgcaggagcg caccatcacc ttcgagggcg atggcaattt caagacccgc gccgaggtga
      361 ccttcgagaa tggcagcgtg tacaatcgcg tgaagctgaa tggccagggc ttcaagaagg
      421 atggccacgt gctgggcaag aatctggagt tcaatttcac cccccactgc ctgtacatct
      481 ggggcgatca ggccaatcac ggcctgaaga gcgccttcaa gatctgccac gagatcaccg
      541 gcagcaaggg cgatttcatc gtggccgatc acacccagat gaataccccc atcggcggcg
      601 gccccgtgca cgtgcccgag taccaccaca tgagctacca cgtgaagctg agcaaggatg
      661 tgaccgatca ccgcgataat atgagcctga aggagaccgt gcgcgccgtg gattgccgca
      721 agacctacct gtgaggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgattt
//
