LOCUS       sequence-72528-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a BASTA resistance cassette as GreenGate module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGF008
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(267..818)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctatagggcg agaattcggt ctcaatactt tatcctagtt tgcgcgctat attttgtttt
       61 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
      121 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
      181 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
      241 aacgatctgc ttgactctag gggtcatcag atttcggtga cgggcaggac cggacggggc
      301 ggcaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
      361 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
      421 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
      481 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctgatg gcggggggag
      541 acgtacacgg tggactcggc cgtccagtcg taggcgttgc gtgccttcca gggacccgcg
      601 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
      661 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
      721 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
      781 gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatcccct cgatcgagtt
      841 gagagtgaat atgagactct aattggatac cgagggatta
//
