LOCUS       sequence-72538-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides linker:BFP as GreenGate C-terminal tag module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGD005
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(864..882)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(865..882)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    896..912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tatagggcga gaattcggtc tcagcagcta cttgtaaagt tcatccattc cattaagtcc
       61 acctccaagt ccagcatatc tagcaacagc aacttcatgc tgctcaacgt aagtctcgtt
      121 gttagcctct ttgatcctct caagcctgta atccacatag tacacaccag gcatcttaag
      181 gttcttagca ggcttcttag acctgtaggt ggtcttgatg ttagcgataa ggtgagatcc
      241 tccaacgagc ttaagagcca tatcgtttct accctcaagt ccaccatcag caggataaag
      301 tgtctcagtg aaagcctccc atccaagagt ctttttctgc atcacaggtc cgttagaggt
      361 gaagttcaca cctctgatct tcacgttgta gatgaggcat ccatcctgaa gagaagtatc
      421 ctgagtagca gtgagaacac caccatcctc gtaagtagta actctctccc aagtgaatcc
      481 ctctgggaaa gattgcttga agaaatcagg gataccctga gtgtggttga tgaaggtctt
      541 agatccgtag aggaatgagg tagcaaggat atcgaaagcg aaaggaagag gtccaccttc
      601 cacaaccttg attctcatgg tctgagttcc ctcataaggc tttccctcac cttcagatgt
      661 gcacttgaaa tggtggttat ccacagtacc ttccatgtag agcttcatgt gcatgttttc
      721 tttgatgagt tcctcaccct ttgaaaccat gcatgcagat cctgaaccag aaccactacc
      781 agaacctgat ccagatccag aggtacctcc tgaaccagaa ccactaccag aacctgatcc
      841 acctgatgag accaagcttc acttctatag tgtcacctaa atagctcgtg tgaaattgtt
      901 atccgctcac aattccacac a
//
