LOCUS       sequence-7254-ex       10363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Salmon P, Kindler V, Ducrey O, Chapuis B, Zubler RH, Trono D
  TITLE     High-level transgene expression in human hematopoietic progenitors 
            and differentiated blood lineages after transduction with improved 
            lentiviral vectors.
  JOURNAL   Blood. 2000 Nov 15. 96(10):3392-8.
  PUBMED    11071633
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2000
            Nov 15. 96(10):3392-8."
COMMENT     Sequence Label: pLOX-EWgfp
FEATURES             Location/Qualifiers
     source          1..10363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             4..637
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    684..809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2113..3290
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2344..3281
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3238..3258
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3363..3372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3369..3371,3372..3374,3375..4085)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3369..3371,3372..3374,3375..4085)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3414..3435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3675..3694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4022..4043
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4165..4181)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4166..4182)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4195..4783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4248..4268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4666..4677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4927..4960)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4980..5160
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5185..5203)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5186..5203)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5493..5512)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5726..5745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5845..5867
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5905..5923)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5991..6095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6096..6164,6165..6956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6314..6333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7127..7715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7616..7635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7869..7886
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7956..7976)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7961..8290
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8141..8276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9424..9489
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9619..9639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10064..10198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10101..10120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10155..10174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
       61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
      181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
      241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
      301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
      361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
      421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
      481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc tttgcaaaga
     2041 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     2101 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2161 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2221 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2281 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2341 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     2401 gtgccttgaa ttacttccac tggctgcagt acgtgattct tgatcccgag cttcgggttg
     2461 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     2521 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     2581 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     2641 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     2701 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     2761 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     2821 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     2881 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     2941 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     3001 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     3061 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     3121 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     3181 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     3241 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattctgc
     3301 agtcgacggt accgcgggcc cgggatccag gcctaagctt acgcgtccta gcgctaccgg
     3361 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3721 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3781 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3841 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3901 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3961 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4021 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4081 acaagtccgg actcagatct cgactagcta gtagctagct agctagtcga gctcaagctt
     4141 cggaattcga tatcaagctt atcgcgatac cgtcgacctc gagggaattc cgataatcaa
     4201 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4261 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4321 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4381 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4441 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4501 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4561 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4621 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4681 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4741 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc
     4801 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4861 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     4921 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     4981 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5041 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5101 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5161 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     5221 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     5281 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     5341 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     5401 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     5461 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     5521 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     5581 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     5641 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     5701 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5761 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5821 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5881 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5941 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6001 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6061 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6121 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6181 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6241 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6301 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6361 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6421 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6481 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6541 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6601 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6661 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6721 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6781 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6841 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6901 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6961 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7021 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7081 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7141 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7201 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7261 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7321 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7381 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7441 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7501 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7561 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7621 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7681 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7741 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7801 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7861 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7921 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     7981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8041 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8101 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8281 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     8341 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     8401 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     8461 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     8521 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     8581 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     8641 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     8701 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     8761 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     8821 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     8881 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     8941 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     9001 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     9061 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     9121 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     9181 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     9241 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     9301 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     9361 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     9421 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     9481 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     9541 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     9601 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     9661 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     9721 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     9781 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     9841 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     9901 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     9961 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    10021 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    10081 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10141 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10201 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10261 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10321 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agt
//
