LOCUS       sequence-72550-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate terminator modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGE000
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..353)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(150..169)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cattagtggt accaccagtg ttgtggcatt gattacctcc tctgcagtta tattccccag
       61 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      121 tccccgataa cggagactgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      181 tccccgatat gcaccactgg gtaaagttca cgggagactt tatctgacag cagacgtgca
      241 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
      301 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catagctgtc
      361 ctcctgagct cttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
      421 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
      481 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
      541 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
      601 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
      661 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
      721 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
      781 atcaccagct caccgtcttt cattgccata cggaactccg gatgagcatt catcaggcgg
      841 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
      901 aaggccgtaa tatccagctg a
//
