LOCUS       sequence-72551-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate N-terminal tag + coding 
            sequence + C-terminal tag modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGI000
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..372)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(169..188)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atagggcgag aattcggtct cagcagggta ccaccagtgt tgtggcattg attacctcct
       61 ctgcagttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
      121 gctgagatca gccacttctt ccccgataac ggagactggc acactggcca tatcggtggt
      181 catcatgcgc cagctttcat ccccgatatg caccactggg taaagttcac gggagacttt
      241 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat
      301 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac
      361 cttaaactgc atagctgtcc tcctgagctc ttacgccccg ccctgccact catcgcagta
      421 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      481 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      541 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      601 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
      661 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      721 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      781 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaactccgg
      841 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tg
//
