LOCUS       sequence-72552-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate N-terminal tag + coding 
            sequence + C-terminal tag + terminator modules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGH000
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..374)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(171..190)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctatagggcg agaattcggt ctcatagtgg taccaccagt gttgtggcat tgattacctc
       61 ctctgcagtt atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg
      121 tggctgagat cagccacttc ttccccgata acggagactg gcacactggc catatcggtg
      181 gtcatcatgc gccagctttc atccccgata tgcaccactg ggtaaagttc acgggagact
      241 ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca
      301 ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa
      361 accttaaact gcatagctgt cctcctgagc tcttacgccc cgccctgcca ctcatcgcag
      421 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
      481 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
      541 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
      601 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
      661 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
      721 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
      781 caagggtgaa cactatccca tatcaccagc tcaccgtctt tc
//
