LOCUS       sequence-7256-ex        9762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-IWgfp
FEATURES             Location/Qualifiers
     source          1..9762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             4..637
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    684..809
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     regulatory      2762..2771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2768..2770,2771..2773,2774..3484)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2768..2770,2771..2773,2774..3484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2813..2834)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3074..3093)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3421..3442
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3564..3580)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3565..3581)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3594..4182
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3647..3667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4065..4076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4326..4359)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4379..4559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4584..4602)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4585..4602)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4892..4911)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5125..5144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5244..5266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5304..5322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5390..5494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5495..5563,5564..6355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5713..5732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6526..7114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7015..7034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7268..7285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7355..7375)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7360..7689
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7540..7675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8823..8888
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9018..9038
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9463..9597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9500..9519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9554..9573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
       61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
      121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
      181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
      241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
      301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
      361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
      421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
      481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataacc actccaagtg
     2041 gaggctgaga aaggttttgt agctgggtag agtatgtact aagagatgga gacagctggc
     2101 tctgagctct gaagcaagca cctcttatgg agagttgctg accttcaggt gcaaatctaa
     2161 gatactacag gagaatacac catgggcttc agcccagttg actcccgagt gggctatggg
     2221 tttgtggaag gagagataga agagaaggga cctttcttct tgaattctgc tttccttcta
     2281 cctctgaggg tgagctgggg tctcagctga ggtgaggaca cagctatcag tgggaactgt
     2341 gaaacaacag ttcaagggac aaagttacta ggtcccccaa caactgcagc ctcctgggga
     2401 atgatgtgga aaaatgctca gccaaggaca aagaaggcct caccctctct gagacaatgt
     2461 cccctgctgt gaactggttc atcaggccac ccaggagccc ctcttaagac tctaattacc
     2521 ctaaggctaa gtagaggtgt tgttgtccaa tgagcacttt ctgcagacct agcaccaggc
     2581 aagtgtttgg aaactgcagc ttcagcccct ctggccatct gctgatccac ccttaatggg
     2641 acaaacagca aagtccaggg gtcagggggg gggtgctttg gactataaag ctagtgggga
     2701 ttcagtaacc cccagcccta aggatccagg cctaagctta cgcgtcctag cgctaccggt
     2761 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2821 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2881 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2941 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3001 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3061 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3121 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3181 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3241 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3301 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3361 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3421 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3481 caagtccgga ctcagatctc gactagctag tagctagcta gctagtcgag ctcaagcttc
     3541 ggaattcgat atcaagctta tcgcgatacc gtcgacctcg agggaattcc gataatcaac
     3601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3841 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3901 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3961 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     4021 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4081 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4141 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg
     4201 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4261 aaggggggac tggaagggct aattcactcc caacgaagac aagatgggat caattcacca
     4321 tgggaataac ttcgtatagc atacattata cgaagttatg ctgctttttg cttgtactgg
     4381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4561 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     4621 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     4681 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     4741 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     4801 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     4861 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4921 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     4981 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5041 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5101 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5161 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5221 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5281 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5341 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5401 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5461 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5521 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5581 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5641 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5701 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5761 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5821 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5881 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5941 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6001 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6061 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6121 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6181 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6241 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6301 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6361 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6421 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6481 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6541 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6601 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6661 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6721 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6781 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6841 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6901 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6961 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7021 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7081 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7141 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7201 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7261 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7321 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7381 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7441 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7501 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7561 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7621 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7681 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     7741 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     7801 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     7861 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     7921 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     7981 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     8041 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     8101 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8161 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8221 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8281 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8341 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8401 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     8461 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     8521 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     8581 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     8641 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     8701 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     8761 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     8821 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     8881 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     8941 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     9001 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     9061 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9121 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9181 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9241 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9301 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9361 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9421 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9481 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9541 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9601 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9661 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9721 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gt
//
