LOCUS       sequence-72568-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for subcloning UPRT into expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Miller MR, Robinson KJ, Cleary MD, Doe CQ
  TITLE     TU-tagging: cell type-specific RNA isolation from intact complex 
            tissues.
  JOURNAL   Nat Methods. 2009 Jun;6(6):439-41. doi: 10.1038/nmeth.1329. Epub 
            2009 May 10.
  PUBMED    19430475
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1329"; journalName: "Nat Methods"; date: "2009-06"; 
            volume: "6"; issue: "6"; pages: "439-41"
COMMENT     Sequence Label: pBS-HA::UPRT
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             67..93
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(890..909)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(891..909)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct
       61 agaatgtacc cctacgatgt gcccgattac gccactagtg ctagcagatc tgcggccaac
      121 atggcgcagg tcccagcgag cggaaagctc cttgtcgatc cccgatattc gacaaacgac
      181 caggaagaaa gcattctcca ggacatcatc acgaggtttc ccaatgtggt gctcatgaag
      241 cagacggctc agcttcgagc gatgatgacc atcattcgtg ataaagaaac accgaaggaa
      301 gaattcgtct tctacgccga ccgcctgatt cgcctcctca tcgaagaagc tttgaacgaa
      361 ctgccgttcg aaaagaagga agtgacaacc cctctggatg tgtcatacca tggagtttcc
      421 ttctattcca agatctgtgg cgtctcgatt gtgagagctg gcgagtcgat ggaaagcggc
      481 ttgcgggcag tttgccgcgg ctgccgcatc gggaaaatcc tcatccagag agacgaaaca
      541 actgcggagc ctaagctgat ctacgagaag ctgcctgccg acattagaga tcgctgggtg
      601 atgctgctag atccgatgtg cgcgacggcg ggcagtgtgt gcaaagcgat cgaggtcctc
      661 ctgaggctcg gcgtgaagga agagagaatc attttcgtca acattttggc tgctccccaa
      721 ggcattgaac gtgttttcaa ggaatacccg aaagtccgca tggtcactgc tgctgttgac
      781 atctgcctga actcgaggta ctacatcgtc cccggcattg gtgatttcgg tgaccggtac
      841 tttggaacca tgtaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
      901 gtcgtattac aattcactgg ccgtcgtttt
//
