LOCUS       sequence-72571-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA left border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGY003
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    224..246
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      373..808
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatagc tgacctccta agcttagcac
       61 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
      121 agcgcgcaaa tacgcatact gttatctggc ttttagtaag cctcgagggt ctcagtattc
      181 tagaccaagc atgcacagct ggcgttctca gccagatcca acgttacacc acaatatatc
      241 ctgccaagat ctaattccgg ggatcggaaa tccagaagcc cgagaggttg ccgcctttcg
      301 ggctttttct ttttcaaaaa aaaaaattta taaaacgatc tgttgcggcc ggccgccggg
      361 ttgtgggcaa aggcgctcga cggtgggcaa ccgcttgcgg ttgtccacgg gcggagccgg
      421 tgcgcgtagc gcattgtcca caagccaagg gcgaccaata attgatatat atattcataa
      481 ttgaaaagct aattgaacat actacttgct gtaactactt gccggagcga ggggtgtttg
      541 caagctgttg atctgaaagg gctattagcg ttctcacgtg cctttttgat tagcgatttc
      601 acgtgacctt attagcgatt tcacgtactc cgattagcga tttcacgtac cctgattagc
      661 gatttcacgt ggatagtttt tggagcgggc cggaaagccc cgtgaatcaa ggctttgcgg
      721 ggcattagcg gtttcacgtg gataactacc ctctatccac aggcttccgg ggataaaaaa
      781 gcccgctcga cggcgggctg ttggatgggg atcgcctgaa tcgccccatc atccagccag
      841 aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg
      901 aacttttgct ttgc
//
