LOCUS       sequence-72572-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate supermodules with plant 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGN000
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     221..240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(486..791)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(588..607)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg tattactgtt
       61 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
      121 tcagagattt tgagacacgg gccagagctg cagctggatg gggtctcaat acctcgagaa
      181 aaaaaagcgg acctccgaag aggtccgcca gatgatcatg cggcatcaga gcagattgta
      241 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      301 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
      361 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      421 acgccagggt tttcccagtc nngacgttgt aaaacgacgg ccagcattga ttacctccta
      481 ctagtttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg
      541 ctgagatcag ccacttcttc cccgataacg gagactggca cactggccat atcggtggtc
      601 atcatgcgcc agctttcatc cccgatatgc accactgggt aaagttcacg ggagacttta
      661 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
      721 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
      781 ttaaactgca tagctgtcct cctgagctct tacgccccgc cctgccactc atcgcagtac
      841 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      901 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat a
//
