LOCUS       sequence-72573-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty entry vector for creating GreenGate supermodules w/o plant 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGM000
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     219..238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     432..454
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(484..789)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(586..605)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgca gctggatggg gtctcaccta ctcgagaaaa
      181 aaaagcggac ctccgaagag gtccgccaga tgatcatgcg gcatcagagc agattgtact
      241 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
      301 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      361 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      421 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agcattgatt acctcctact
      481 agtttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      541 gagatcagcc acttcttccc cgataacgga gactggcaca ctggccatat cggtggtcat
      601 catgcgccag ctttcatccc cgatatgcac cactgggtaa agttcacggg agactttatc
      661 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      721 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
      781 aaactgcata gctgtcctcc tgagctctta cgccccgccc tgccactcat cgcagtactg
      841 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      901 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaacg
      961 g
//
