LOCUS       sequence-72574-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA left border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGZ003
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    225..247
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      374..809
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccatag ctgacctcct aagcttagca
       61 cacctctttt tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat
      121 cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gcctcgaggg tctcagtatt
      181 ctagaccaag catgcacagc tggcgttctc agccagatcc aacgttacac cacaatatat
      241 cctgccaaga tctaattccg gggatcggaa atccagaagc ccgagaggtt gccgcctttc
      301 gggctttttc tttttcaaaa aaaaaaattt ataaaacgat ctgttgcggc cggccgccgg
      361 gttgtgggca aaggcgctcg acggtgggca accgcttgcg gttgtccacg ggcggagccg
      421 gtgcgcgtag cgcattgtcc acaagccaag ggcgaccaat aattgatata tatattcata
      481 attgaaaagc taattgaaca tactacttgc tgtaactact tgccggagcg aggggtgttt
      541 gcaagctgtt gatctgaaag ggctattagc gttctcacgt gcctttttga ttagcgattt
      601 cacgtgacct tattagcgat ttcacgtact ccgattagcg atttcacgta ccctgattag
      661 cgatttcacg tggatagttt ttggagcggg ccggaaagcc ccgtgaatca aggctttgcg
      721 gggcattagc ggtttcacgt ggataactac cctctatcca caggcttccg gggataaaaa
      781 agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat catccagcca
      841 gaaagtgagg gagccacggt tgatga
//
