LOCUS       sequence-72576-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to make an RNA probe for whole-mount in situ 
            hybridization, for in-frame fusion proteins or as a subcloning step 
            for mRFP. Note that the coding sequence for mRFP lacks the start 
            codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, 
            Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, 
            Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
  TITLE     In vivo protein trapping produces a functional expression codex of 
            the vertebrate proteome.
  JOURNAL   Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
  PUBMED    21552255
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "506-15. Epub 
            2011 May 8"
COMMENT     Sequence Label: pCR4-*mRFP
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(84..102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(109..126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(109..125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(118..140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             263..565
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     444..463
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtacgagcgc gccgagggcc gccactccac cggagcctaa gactagttta agggcgaatt
       61 cgcggccgct aaattcaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcgcagc ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta
      301 tcgtctgttt gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat
      361 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
      421 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
      481 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
      541 taacctgatg ttctggggaa tataaatgtc aggcatgaga ttatcaaaaa ggatcttcac
      601 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
      661 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
      721 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
      781 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
      841 tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg
      901 aggatcgttt cgcatgattg aacaagatgg attgcacgc
//
