LOCUS       sequence-72577-e         556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7b
FEATURES             Location/Qualifiers
     source          1..556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..25
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggacctaag aaaaagagga aggtggcggc cgctgactac aaggatgacg acgataaacc
       61 aggtggcgga ggtagtggcg gaggtggggt acccgccagt ccagcagccc aggtggatct
      121 gagaaccctc ggctacagcc agcagcagca ggagaagatc aaaccaaagg tgcggtccac
      181 cgtcgctcag caccatgaag cactggtggg gcacggtttc acacacgccc atattgtggc
      241 tctgtctcag catcccgctg cactcgggac tgtggccgtc aaatatcagg acatgatcgc
      301 cgctctgcct gaggcaaccc acgaagccat tgtgggcgtc ggaaagcagt ggagcggtgc
      361 cagagcactc gaagcactcc tcaccgtcgc cggggaactg cggggtccac cactccagtc
      421 cggactggac actggacagc tgctgaagat cgctaaacgc ggcggagtga cagctgtgga
      481 agctgtgcac gcttggagga atgctctgac aggagccccc ctggagacgg gcgccgctac
      541 agggcgcgtc ccattc
//
