LOCUS       sequence-72578-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Pimephales notatus cryaa
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             591..608
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(661..679)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      675..722
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(846..865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatggatat tgccatccaa cacccctggt tcaaacgcaa
       61 catgggctac cccacccgtc tcttcgacca gttctttgga gaaggcctgt tcgattatga
      121 cctcttcccc ttcgctccct caactattag cccctactat cgacattcac tcttccgcaa
      181 cttcctggac tcctccaact caggcatctc tgaggtgagg tctgacagag acaaattcac
      241 agtgtacctg gatgtgaaac acttctctcc tgaagagctc aatgtcaagg tgacagatga
      301 ctatgtggag atcgagggca agcatgcaga aagacaggat gatcatggct acatctcccg
      361 tgagttccac cgtcgctacc gtctgccttc caacgtggac cagtctgcca tccactgctc
      421 actgactgct gatggcctac ttactctttg tggacccaag actggtggca tagatgctgg
      481 ccgcggagac cgcaccatcc ctgttacccg tgaggacaag accaactcag gctcctcctc
      541 ctagggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
      601 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      661 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      721 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      781 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      841 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccc
//
