LOCUS       sequence-72596-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min 
            H, Golden SS
  TITLE     Application of bioluminescence to the study of circadian rhythms in 
            cyanobacteria.
  JOURNAL   Methods Enzymol. 2000;305:527-42.
  PUBMED    10812624
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2000"; volume: "305"; pages: "527-42"
COMMENT     Sequence Label: pAM1573
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(330..989)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     904..923
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcggattttg catcacgaag cgggtcacta cttggtagca accgcgctgg ggttaccaga
       61 tccgtcgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
      121 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
      181 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
      241 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
      301 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
      361 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      421 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      481 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      541 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      601 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      661 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      721 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
      781 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      841 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      901 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      961 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1021 aactcaaaaa atacgcccgg
//
