LOCUS       sequence-72598-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7b
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..66
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     58..75
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     59..75
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(151..167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(151..167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(164..186)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(199..205,206..223,224..229))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    244..265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             442..1029
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(1050..1066)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1050..1066)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
       61 aaaacgacgg ccagtgagcg cgcggcgaat tgggtaacgg gccacccctc gaggtcctcc
      121 agcttttgtt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      181 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      241 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccaccggtc
      301 gtctccaacg atcatctggt cgctctcgct tgcctcggtg gacggcccgc tctggacgca
      361 gtcaaaaagg gtctccccca tgctcccgca ctgatcaaga gaaccaacag gagaattcct
      421 gagggatccg atcgtttaaa ccagctcgtg aaaagcgaac tcgaagaaaa gaaaagtgaa
      481 ctgcggcaca aactgaaata cgtcccacat gaatacattg agctgatcga gattgctagg
      541 aactccaccc aggacagaat cctcgagatg aaagtgatgg aattctttat gaaagtctac
      601 gggtatcggg gcaagcacct gggcggatct cgcaaaccag atggggcaat ctacactgtg
      661 ggtagtccca tcgactatgg cgtgattgtc gataccaagg cctacagtgg gggttataat
      721 ctgcccattg gacaggctga cgagatgcag cgatacgtgg aggaaaacca gacaagaaat
      781 aagcatatca accccaatga gtggtggaaa gtgtatccta gctccgtcac tgaattcaag
      841 tttctcttcg tgtcaggcca ctttaaggga aactacaaag cacagctgac caggctcaat
      901 catattacaa actgcaatgg cgccgtgctg agcgtcgagg aactgctcat cggcggagag
      961 atgatcaagg ccggcacact caccctggag gaggtccgcc gaaaattcaa taacggggaa
     1021 atcaacttct gaacgcgtaa atgattgcag atccactagt tctagaattc cagctgagcg
     1081 ccggtcgcta ccattaccag ttggtctgg
//
