LOCUS       sequence-72599-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7b
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        99..146
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             165..563
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             650..883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
       61 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
      121 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagcctttgt
      181 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
      241 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
      301 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
      361 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
      421 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
      481 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
      541 ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag accaatgact
      601 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
      661 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
      721 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      781 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      841 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
      901 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gag
//
