LOCUS       sequence-7260-ex       10242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Wiznerowicz M, Trono D
  TITLE     Conditional suppression of cellular genes: lentivirus 
            vector-mediated drug-inducible RNA interference.
  JOURNAL   J Virol. 2003 Aug . 77(16):8957-61.
  PUBMED    12885912
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2003 Aug . 77(16):8957-61."
COMMENT     Sequence Label: pLVTH-siGFP
FEATURES             Location/Qualifiers
     source          1..10242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2033..2150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2206..2417
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    3419..4007
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3472..3492)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3890..3901)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(4200..4218,4219..4241,4242..4260,4261..4283,4284..4302,
                     4303..4325,4326..4344,4345..4367,4368..4386,4387..4409,
                     4410..4428,4429..4451,4452..4470)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4499..4713
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4634..4653
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(4804..4837)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4857..5037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5062..5080)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5063..5080)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5370..5389)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5603..5622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5722..5744
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5782..5800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5868..5972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5973..6041,6042..6833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6191..6210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7004..7592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7493..7512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7746..7763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7833..7853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7838..8167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8018..8153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9301..9366
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9496..9516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9941..10075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9978..9997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10032..10051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga
     2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
     2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2401 ttgccgccag aacacaggtg tcgtgacgcg gatcgtaccc gccgcggcca gctccggcgg
     2461 gcaggggggg cgctggagcg cagcgcagcg cagccccatc agtccgcaaa gcggaccgag
     2521 ctggaagtcg agcgctgccg cgggaggcgg gcgatggggg caggtgccac cggccgcgcc
     2581 atggacgggc cgcgcctgct gctgttgctg cttctggggg tgtcccttgg aggtgccaag
     2641 gaggcatgcc ccacaggcct gtacacacac agcggtgagt gctgcaaagc ctgcaacctg
     2701 ggcgagggtg tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc ctgcctggac
     2761 agcgtgacgt tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg caccgagtgc
     2821 gtggggctcc agagcatgtc ggcgccgtgc gtggaggccg acgacgccgt gtgccgctgc
     2881 gcctacggct actaccagga tgagacgact gggcgctgcg aggcgtgccg cgtgtgcgag
     2941 gcgggctcgg gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg cgaggagtgc
     3001 cccgacggca cgtattccga cgaggccaac cacgtggacc cgtgcctgcc ctgcaccgtg
     3061 tgcgaggaca ccgagcgcca gctccgcgag tgcacacgct gggccgacgc cgagtgcgag
     3121 gagatccctg gccgttggat tacacggtcc acacccccag agggctcgga cagcacagcc
     3181 cccagcaccc aggagcctga ggcacctcca gaacaagacc tcatagccag cacggtggca
     3241 ggtgtggtga ccacagtgat gggcagctcc cagcccgtgg tgacccgagg caccaccgac
     3301 aacctcatcc ctgtctattg ctccatcctg gctgctgtgg ttgtgggcct tgtggcctac
     3361 atagccttca agaggtggaa cagggggatc ctctagtcga cctcgaggga attccgataa
     3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3961 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcgc
     4021 tcaagcttcg aattaattct gcagagctcg gtacctttaa gaccaatgac ttacaaggca
     4081 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4141 caacgaagac aagatgggat caattcacca tgctagtgga tccccctcga gtttaccact
     4201 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4261 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     4321 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     4381 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     4441 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcggggctgc aggaattcga
     4501 acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca
     4561 cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg
     4621 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga
     4681 tttgggaatc ttataagttc tgtatgagac cacagatccc cgaacggcat caaggtgaac
     4741 ttcaagagag ttcaccttga tgccgttctt tttggaaaag cttatcgata ccgtcgcatg
     4801 ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt
     4861 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4921 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4981 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     5041 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     5101 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     5161 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     5221 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     5281 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     5341 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     5401 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     5461 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     5521 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     5581 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5641 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5701 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5761 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5821 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6181 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7141 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7381 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7801 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     7861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7921 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7981 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8041 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8101 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8161 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     8221 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     8281 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     8341 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     8401 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     8461 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     8521 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     8581 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     8641 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     8701 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     8761 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     8821 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     8881 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     8941 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     9001 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     9061 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     9121 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     9181 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     9241 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     9301 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     9361 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     9421 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     9481 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     9541 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     9601 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     9661 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     9721 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     9781 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     9841 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     9901 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     9961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10081 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10141 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10201 aaagaaattg tatttgttaa atatgtacta caaacttagt ag
//
