LOCUS       sequence-72600-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7a
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..760)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      781..790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgcatgttag aagacttcct ctgccctcgc gagatccggt ggagccgggt ccggcggtgc
       61 cgtccacggc agaattggac gactgagcgc gggatctggc gaaggcgatg ggggtcttga
      121 aggcgtgctg gtactccacg atgcccagct cggtgttgct gtgcagctcc tccacgcggc
      181 ggaaggcgaa catggggccc ccgttctgca ggatgctggg gtggatggcg ctcttgaagt
      241 gcatgtggct gtccaccacg aagctgtagt agccgccgtc gcgcaggctg aaggtgcggg
      301 cgaagctgcc caccagcacg ttatcgccca tggggtgcag gtgctccacg gtggcgttgc
      361 tgcggatgat cttgtcggtg aagatcacgc tgtcctcggg gaagccggtg cccaccacct
      421 tgaagtcgcc gatcacgcgg ccggcctcgt agcggtagct gaagctcacg tgcagcacgc
      481 cgccgtcctc gtacttctcg atgcgggtgt tggtgtagcc gccgttgttg atggcgtgca
      541 ggaaggggtt ctcgtagccg ctggggtagg tgccgaagtg gtagaagccg tagcccatca
      601 cgtggctcag caggtagggg ctgaaggtca gggcgccttt ggtgctcttc atcttgttgg
      661 tcatgcggcc ctgcttgggg gtgccctctc cgccgcccac cagctcgaac tccacgccgt
      721 tcagggtgcc ggtgatgcgg cactcgatct ccatggcggg caggccgctc tcgtcgctct
      781 ccatggtggc gtctagcgta ggcgccggtc acagcttgga tctgtaacgg cgcagaacag
      841 aaaacgaaac aaagacgtag agttgagcaa gcagggtcag gcaaagcgtg gagagccggc
      901 tgagtctagg taggctccaa gggagcgccg gacaaaggcc cggtctcgac ctgagcttta
      961 aacttaccta gacggcggac
//
