LOCUS       sequence-72601-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-SOK
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..117
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(145..204)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 ttccaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt
       61 ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag
      121 gtccctcgac gaattcggcg cgccgggacc tgggttgctc tcaacatctc cagccaattt
      181 caagagagca taattagtac actggcccga gccctcgagt taaaagtgcc tcttcatgtg
      241 taaggcaagg tggtccgacc tggaaaaggc cctgtcgcac ttctggcact gaaagggccg
      301 gtgccctgtg tgtttgcggt agtgcctggt cagttcatcg gagcgggcga atttccaccc
      361 acagccgtcc cagtcacagt ggtaaggttt ctcgcctgtg tgagttcgca ggtgtgcctt
      421 gagatgagaa ctcttggtat aggttttgcc acagcctgca tagtcacaag tgtgggtggc
      481 tgttcttttc cggggccacg accttcttcc cctctttggc ttgggctcct ctggcaggca
      541 ggaacccggt ggcatgagct cttgataatg gagagagggg acttgtgact gcatctggtc
      601 tggcaggaaa ggagggtagt tgggccccgg atggggatgg aatcctgggg gaagaggcag
      661 gccagggtga cagtccctgc tgttcagcag ttcctcggga ctcagtgtag gggtagtcct
      721 ggtggggagc tgccgcccca gggggaagtc gtgtgtgttg ggccggtggc cgttgctgag
      781 ctggggtcca gcgctcaaat gggcctctag ggaccggctg accgtgcagg
//
