LOCUS       sequence-72625-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Frank S, Skryabin BV, Greber B
  TITLE     A modified TALEN-based system for robust generation of knock-out 
            human pluripotent stem cell lines and disease models.
  JOURNAL   BMC Genomics. 2013 Nov 9;14(1):773.
  PUBMED    24206569
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2013-11-9"; volume: "14(1)"; pages: "773"
COMMENT     Sequence Label: pTAL7a
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     53..70
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     54..70
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(146..162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(146..162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(159..181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    170..186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(194..200,201..218,219..224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    239..260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
       61 gacggccagt gagcgcgcgg cgaattgggt aacgggccac ccctcgaggt cctccagctt
      121 ttgttgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      181 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      241 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccac cggtcgtctc
      301 caacgatcat ctggtcgctc tcgcttgcct cggtggacgg cccgctctgg acgcagtcaa
      361 aaagggtctc ccccatgctc ccgcactgat caagagaacc aacaggagaa ttcctgaggg
      421 atccgatcgt ttaaaccagc tcgtgaaaag cgaactcgaa gaaaagaaaa gtgaactgcg
      481 gcacaaactg aaatacgtcc cacatgaata cattgagctg atcgagattg ctaggaactc
      541 cacccaggac agaatcctcg agatgaaagt gatggaattc tttatgaaag tctacgggta
      601 tcggggcaag cacctgggcg gatctcgcaa accagatggg gcaatctaca ctgtgggtag
      661 tcccatcgac tatggcgtga ttgtcgatac caaggcctac agtgggggtt ataatctgcc
      721 cattggacag gctgacgaga tgcagcgata cgtggaggaa aaccagacaa gaaataagca
      781 tatcaacccc aatgagtggt ggaaagtgta tcctagctcc gtcactgaat tcaagtttct
      841 cttcgtgtca ggccacttta a
//
