LOCUS       sequence-72631-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for NM with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-NM
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    132..213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     157..176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(496..519)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(694..713)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttta ttaccgcctt
       61 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      121 ggaagctcga ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      181 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      241 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      301 ggtatggctg attatgatcc tctagagata tcgtcgacaa gcttatcgat gcggccgcgc
      361 tagcacgcgt cagctgacta gaggatcccc gggtaccgag ctcgaattct tacttgtaca
      421 gctcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg tactgttcca
      481 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg tagtagtagc
      541 cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc aggtagtggc
      601 cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg tcgcgggggt
      661 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
      721 gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc tgcagggagg
      781 agtcctgggt cacggtcacc agaccgccgt cctcgaagtt catcacgcgc tcccacttga
      841 agccctcggg gaaggacagc ttcttgtaat cggggatgtc ggcggggtgc ttcacgtacg
      901 ccttggagcc gtacatgaac tgggg
//
