LOCUS       sequence-72632-e        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of TALEN proteins with FokI-RRR domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Hu R, Wallace J, Dahlem TJ, Grunwald DJ, O'Connell RM
  TITLE     Targeting human microRNA genes using engineered Tal-effector 
            nucleases (TALENs).
  JOURNAL   PLoS One. 2013 May 7;8(5):e63074. doi: 10.1371/journal.pone.0063074.
            Print 2013.
  PUBMED    23667577
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-7"; volume: "8(5):e63074. doi"; pages: " 
            10.1371/journal.pone.0063074. Print 2013"
COMMENT     Sequence Label: pCS2TAL3-RRR
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             146..235
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(548..955)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     805..827
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     819..836
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     820..836
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     846..865
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        846..864
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(912..932)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(912..930)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(951..967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(951..967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(964..986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(999..1005,1006..1023,1024..1029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1044..1065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ctgccaccat
      121 ggctccaaag aagaagcgta aggtataccc atacgatgtt cctgactatg cgggctatcc
      181 ctatgacgtc ccggactatg caggatcgta tccatatgac gttccagatt acgctgctca
      241 tggtaccgtg gatctacgca cgctcggcta cagtcagcag cagcaagaga agatcaaacc
      301 gaaggtgcgt tcgacagtgg cgcagcacca cgaggcactg gtgggccatg ggtttacaca
      361 cgcgcacatc gttgcgctca gccaacaccc ggcagcgtta gggaccgtcg ctgtcacgta
      421 tcagcacata atcacggcgt tgccagaggc gacacacgaa gacatcgttg gcgtcggcaa
      481 acagtggtcc ggcgcacgcg ccctggaggc cttgctcacg gatgcggggg agttgagagg
      541 tccgccgtta cagttggaca caggccaact tgtgaagatt gcaaaacgtg gcggcgtgac
      601 cgcaatggag gcagtgcatg catcgcgcaa tgcactgacg ggtgcccccc tggagacggg
      661 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
      721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
      781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
      841 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc
      901 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
      961 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1021 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1081 ctgcccgctt tccaccggtc gtctcca
//
