LOCUS       sequence-72635-e         389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for NM with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-NM
FEATURES             Location/Qualifiers
     source          1..389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        143..181
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     178..202
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             225..248
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgactcacta tagggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga
       61 tggatatctg cagaattcgc ccttgcgacg tagggataac agggtaatag tgtcccctcc
      121 accccacagt gggaggattc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      181 tcgtttagtg aaccgtcaga tcgcctggag aattcgccac catggactac aaggatgacg
      241 acgataaaac ttccggtggc ggactgggtt ccaccgtgag caagggcgag gaggtcatca
      301 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      361 tcgagggcga gggcgagggc cgcccctac
//
