LOCUS       sequence-72637-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null SP Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcasN-
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctgttttga atggttccaa caagattatt ttataacatt tataacaaat aatcaaggag
       61 aaattcaaag aaatttatca gccggttagt ctccaccgag ctgagagagg tcgattcttg
      121 tttcatagag ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag
      181 aggtgtaccg ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca
      241 agttggtcag agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc
      301 tgtgcttatt gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt
      361 tggagaattc gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga
      421 acagctgctt ctgctcatta tcttcgggag accctttgag cttttcatag tggctggcca
      481 gatacaagaa attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc
      541 ccgcactagc gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa
      601 gcttaatgat gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg
      661 ggtttttttc gaagcttgat cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt
      721 tgagtttttt agacttccct ttctccactt tggccacaac cagtacactg taagcgactg
      781 taggagaatc gaatccgccg tatttcttgg ggtcccaatc ttttttgcgt gcgatcagct
      841 tgtcgctgtt ccttttcggg aggatacttt ccttggagaa gcctccggtc tgtacttcgg
      901 tctttttaac gatgttcacc tgcggcatgg acaggacctt ccggactgtc gcgaaatccc
      961 taccct
//
