LOCUS       sequence-72640-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian S. pyogenes Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SPcas
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg gactctagag gatcgaaccc ttgccaccat
      121 ggacaagaag tactccattg ggctcgatat cggcacaaac agcgtcggct gggccgtcat
      181 tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca
      241 cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc
      301 cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta
      361 cctgcaggag atctttagta atgagatggc taaggtggat gactctttct tccataggct
      421 ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa
      481 tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa
      541 gcttgtagac agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat
      601 gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt
      661 cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat
      721 caacgcatcc ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg
      781 gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct
      841 tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga
      901 tgccaagctt caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca
      961 gatcggcgac cagtacg
//
