LOCUS       sequence-72642-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcas
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttccaa caagattatt ttataacatt tataacaaat aatcaaggag aaattcaaag
       61 aaatttatca gccggtcagt cacctcctag ctgactcaaa tcaatgcgtg tttcataaag
      121 accagtgatg gattgatgga taagagtggc atctaaaact tcttttgtag acgtatatcg
      181 tttacgatca attgttgtat caaaatattt aaaagcagcg ggagctccaa gattcgtcaa
      241 cgtaaataaa tgaataatat tttctgcttg ttcacgtatt ggtttgtctc tatgtttgtt
      301 atatgcacta agaactttat ctaaattggc atctgctaaa ataacacgct tagaaaattc
      361 actgatttgc tcaataatct catctaaata atgcttatgc tgctccacaa acaattgttt
      421 ttgttcgtta tcttctggac tacccttcaa cttttcataa tgactagcta aatataaaaa
      481 attcacatat ttgcttggca gagccagctc atttcctttt tgtaattctc cggcactagc
      541 cagcatccgt ttacgaccgt tttctaactc aaaaagacta tatttaggta gtttaatgat
      601 taagtctttt ttaacttcct tatatccttt agcttctaaa aagtcaatcg gatttttttc
      661 aaaggaactt ctttccataa ttgtgatccc tagtaactct ttaacggatt ttaacttctt
      721 cgatttccct ttttccacct tagcaaccac taggactgaa taagctaccg ttggactatc
      781 aaaaccacca tatttttttg gatcccagtc ttttttacga gcaataagct tgtccgaatt
      841 tctttttggt aaaattgact ccttggagaa tccgcctgtc tgtacttctg ttttcttgac
      901 aatattgact tggggcatgg acaatacttt gcgcactgtg gcaaa
//
