LOCUS       sequence-72645-e        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian S. pyogenes Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SPcas
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..52)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attactaacc ggtagggatc gaaccctttc acaccttcct cttcttcttg gggtcagccc
       61 tgctgtctcc accgagctga gagaggtcga ttcttgtttc atagagcccc gtaattgact
      121 gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt ctgtctatgg
      181 tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta aacaagtgga
      241 taatgttttc tgcctgctcc ctgatgggct tatccctgtg cttattgtaa gcagaaagca
      301 ccttatcgag gttagcgtcg gcgaggatca ctcttttgga gaattcgctt atttgctcga
      361 tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc tcattatctt
      421 cgggagaccc tttgagcttt tcatagtggc tggccagata caagaaatta acgtatttag
      481 agggcagtgc cagctcgtta cctttctgca gctcgcccgc actagcgagc attcgtttcc
      541 ggccgttttc aagctcaaag agagagtact tgggaagctt aatgatgagg tcttttttga
      601 cctctttata tcctttcgcc tcgagaaagt cgatggggtt tttttcgaag cttgatcgct
      661 ccatgattgt gatgcccagc agttccttga cgcttttgag ttttttagac ttccctttct
      721 ccactttggc cacaaccagt acactgtaag cgactgtagg agaatcgaat ccgccgtatt
      781 tcttggggtc ccaatctttt ttgcgtgcga tcagcttgtc gctgttcctt ttcgggagga
      841 tactttcctt ggagaagcct ccggtctgta cttcggtctt tttaacgatg ttcacctgcg
      901 gcatggacag gaccttccgg actgtcgcga aatccctacc cttgtcccac acgatttctc
      961 ctgtttctcc gtttgtttcg ataagtggtc gcttccgaat ctctccattg gccagtgtaa
     1021 tctcggtctt gaaaaaattc ata
//
