LOCUS       sequence-72651-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian S. thermophilus #1 Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-ST1cas
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg gactctagag gatcgaaccc ttgccaccat
      121 gagcgacctg gtgctgggcc tggacatcgg catcggcagc gtgggcgtgg gcatcctgaa
      181 caaggtgacc ggcgagatca tccacaagaa cagtcgcatc ttccctgctg ctcaggctga
      241 gaacaacctg gtgcgccgca ccaaccgcca gggtcgccgg cttgctcgcc gcaagaagca
      301 ccggcgcgtg cgcctgaacc gcctgttcga ggagagcggc ctgatcaccg acttcaccaa
      361 gatcagcatc aacctgaacc cctaccagct gcgcgtgaag ggcctgaccg acgagctgag
      421 caacgaggag ctgttcatcg ccctgaagaa catggtgaag caccgcggca tcagctacct
      481 ggacgacgcc agcgacgacg gcaacagcag cgtgggcgac tacgcccaga tcgtgaagga
      541 gaacagcaag cagctggaga ccaagacccc cggccagatc cagctggagc gctaccagac
      601 ctacggccag ctgcgcggcg acttcaccgt ggagaaggac ggcaagaagc accgcctgat
      661 caacgtgttc cccaccagcg cctaccgcag cgaggccctg cgcatcctgc agacccagca
      721 ggagttcaac ccccagatca ccgacgagtt catcaaccgc tacctggaga tcctgaccgg
      781 caagcgcaag tactaccacg gccccggcaa cgagaagagc cgcaccgact acggccgcta
      841 ccgcaccagc ggcgagaccc tggacaacat cttcggcatc ctgatcggca agtgcacctt
      901 ctaccccgac gagttccgcg ccgccaaggc cagctacacc gcccaggagt tcaacctgct
      961 ga
//
