LOCUS       sequence-72658-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian S. thermophilus #1 Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-ST1cas
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..53)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attactaacc ggtagggatc gaaccctgct cacaccttcc tcttcttctt ggggtcagcc
       61 ctgctgaagt ccaacttggg cttgtcgccc tcgttcttga tgatgtgctg gttgcccagc
      121 acgtcggtgc gcaccttgta gatgctgatg ttgctcttgc ccaggccctt cttgcactgg
      181 ccgctgttgg ccacgttgcc cagcaccttg atcagggcct cgccgccctc gaacttctgc
      241 ttgtcgtagg gcttcagctc cacgtagtgc ttctgcttgg gcatggtgcg gctcaggaag
      301 cggaacagct gttgctcctt ggtctcggtg tccttcacca gcagaaggtc gttcttgtac
      361 agggtgaact tgaactcgct gtcgctgtcc acgccctcct tcttcttgat gtcgttgtac
      421 ttctcctggc tgatcttgta ggtgccggtg cccttatcaa actgcaggtc ggcgtacttc
      481 aggcccagga tctcgtactt gccggtggtc ttgttgaagt acacgtcggc gcgccagggg
      541 ctcacgctct gcagcaccac cttgttgttg ctgtccttgg gggtgatgtc gatgtggttg
      601 cccagcttgc tgtcgtagta cttcaggctc ttgatctcgg ggccgttgcc cttcttgctg
      661 tacttgcgga tgtagccgtg ctcctccttg tacttcagga aggggttgca gggcacctcc
      721 ttgcctttat cgttgatctg cttgttgggg tagttctcca ggatgggctc gatcaccttc
      781 tcgaaggtct gggggtcgtg gcggtacatc aggaacttgc tcttgtcctt cttgtagatc
      841 ttcatgaagg cgtcgtagcc gtcctgggtg tagatgtcct tgatcttgcc cagcacgtag
      901 gtctcgtcgg ccttgtcctt gcccaccttg gcctggcggg tggcgta
//
