LOCUS       sequence-72660-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of TALEN proteins with FokI-DDD domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Hu R, Wallace J, Dahlem TJ, Grunwald DJ, O'Connell RM
  TITLE     Targeting human microRNA genes using engineered Tal-effector 
            nucleases (TALENs).
  JOURNAL   PLoS One. 2013 May 7;8(5):e63074. doi: 10.1371/journal.pone.0063074.
            Print 2013.
  PUBMED    23667577
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-7"; volume: "8(5):e63074. doi"; pages: " 
            10.1371/journal.pone.0063074. Print 2013"
COMMENT     Sequence Label: pCS2TAL3-DDD
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..55)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(39..55)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(52..74)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    63..79
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    132..153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             339..926
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQD
                     YVEENQTRDKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
       61 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      181 caccggtcgt ctccaacgac cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca
      241 tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc
      301 gtattggcga acgcacgtcc catcgcgttg ccggatccca actagtcaaa agtgaactgg
      361 aggagaagaa atctgaactt cgtcataaat tgaaatatgt gcctcatgaa tatattgaat
      421 taattgaaat tgccagaaat tccactcagg atagaattct tgaaatgaag gtaatggaat
      481 tttttatgaa agtttatgga tatagaggta aacatttggg tggatcaagg aaaccggacg
      541 gagcaattta tactgtcgga tctcctattg attacggtgt gatcgtggat actaaagctt
      601 atagcggagg ttataatctg ccaattggcc aagcagatga aatgcaagac tatgtcgaag
      661 aaaatcaaac acgagacaaa catatcaacc ctaatgaatg gtggaaagtc tatccatctt
      721 ctgtaacgga atttaagttt ttatttgtga gtggtcactt taaaggaaac tacaaagctc
      781 agcttacacg attaaatcat atcactaatt gtaatggagc tgttcttagt gtagaagagc
      841 ttttaattgg tggagaaatg attaaagccg gcacattaac cttagaggaa gtgcgccgga
      901 aatttaataa cggcgagata aacttttaat ctagaactat agtgagtcgt attacgtaga
      961 tccagacatg ataagataca ttgatga
//
